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Download Biochemistry Protocols Links (Updated):

Free Download Biochemistry Protocols in PDF

Qualitative Analysis:

  1. Color reactions of Amino acids
  2. Color Reactions of Carbohydrates
  3. Color reactions of Lipids
  4. Color reactions of Proteins
  5. Color reactions of Albumins and Globulins

Quantitative Analysis:

Colorimetric Analysis:

  1. Anthrone Method
  2. BRADFORD PROTEIN ASSAY
  3. Lowry Method
  4. Biuret Method
  5. Estimation of Pyruvate method by using 2,4-DNP method
  6. FC Method
  7. DNS method
  8. Fiske-Subbarao method

Titrimetric Analysis:

  1. Determination of Pi of Glycine
  2. Estimation of Calcium by EDTA method
  3. Pi and Pka analysis of Amino acids by Titrimetric method
  4. Saponification value of Fats
  5. Benedict’s method
  6. Formal Titration method
  7. Ascorbic acid estimation by 2,6-dichlorophenol
  8. Saponification value
  9. Food Science – Nutrition
  10. Statch isolation from Potatoes
  11. Casein from Milk
  12. Phosphorous determination in wheat flour
  13. Ca+2 estimation in wheat flour

Enzymology :

  1. Assay of Salivary amylase enzyme activity
  2. Effect of pH on Enzyme activity
  3. Effect of Temperature on amylase activity
  4. Catalase Activity
  5. Urease activity
  6. Effect of Temperature on amylase activity
  7. Time course of enzyme activity (Salivary amylase)
  8. Effect of substrate concentration on amylase activity and determination of Km constant
  9. Assay of acid phosphatase from potatoes
  10. Effect of substrate concentration on acid phosphatase activity
  11. Effect of substrate concentration on Urease activity
  12. Determination of Trypsin activity
  13. Effect of Metal ions on amylase activity

 Biochemical Techniques:

  1. Purification of Proteins by Affinity chromatography
  2. Purification of enzyme by ammonium sulfate fractionation

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