Category Archives for "Biochemical Techniques"

Spectrophotometer Instrumentation : Principle and Applications

What are the basic components of Spectrophotometer instrumentation? What is Electromagnetic radiation? Electromagnetic radiation has been put to many uses in our daily routine. The radio and television broadcasting, medical x-ray etc are some common examples. The use of electromagnetic radiation in analytical chemistry gained much importance during the last 50 years for characterization of […]

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What is Ion Exchange Chromatography and its Applications

W.Cohn first developed this procedure.  The reversible exchange of ions in solution with ions electrostatically bound to some sort of insoluble support medium. Basics of Biochemical Techniques What is Paper Chromatography? Principle and Procedure Ninhydrin test or Ninhydrin reagent Ion exchange chromatography definition (or ion chromatography) is a process that allows the separation of ions […]

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What is Paper Chromatography? Principle and Procedure

Paper chromatography has proved to be very successful in the analysis of chemical compound and lipid sample in particular. In paper chromatography, the sample mixture is applied to a piece of filter paper, the edge of the paper is immersed in a solvent, and the solvent moves up the paper by capillary action. What is […]

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Special Notes on Spectrophotometer Calibration

What is A Spectrophotometer? A spectrophotometer can be located in many study, biology, chemistry, and industrial laboratories. Spectrophotometer are utilized for research and data evaluation in different scientific fields. Some of the major fields in which a spectrophotometer is employed is physics, molecular biology, chemistry, and biochemistry labs. Generally, the title refers to Ultraviolet-Visible (UV-Vis) […]

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