Fatty acids are the Building Blocks of Fats and Oils (Short Notes)

Do you know fatty acids are the basic unit of which macromolecules? Fats are esters of high molecular weight containing Fatty acids(FA) and Glycerol. Glycerol is a trihydric alcohol and therefore one two or all the three hydroxyl groups (OH) can react with FA forming mono, di or triglycerides respectively. These are the basic questions. To solve […]

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  • Updated April 8, 2019

Pyrimidine Synthesis Pathway: Synthesis of pyrimidine derivatives

The pyrimidine synthesis is a similar process than that of Purines(Purines Synthesis). In the de novo synthesis of Pyrimidines, the ring is synthesized first and then it is attached to a ribose-phosphate to for a pyrimidine nucleotide. Pyrimidine rings are assembled from bicarbonate, aspartate, and Ammonia. The pyrimidine biosynthesis (de novo pyrimidine synthesis pathway) was […]

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  • Updated April 18, 2019

Estimation of Blood Glucose level by Folin-Wu method

Estimation of Blood Glucose level by Folin-Wu method is one of the clinical biochemistry protocol. You can download these protocols from the given link. Table of Contents What is Folin-Wu tube?Estimation of Blood Glucose level by Folin-Wu methodAim: Principle:Reagents: Procedure:Deproteination of Blood:Normal values:Report:Calculation:CLINICAL SIGNIFICANCE: What is Folin-Wu tube? The modification of the Folin and Wu […]

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  • Updated April 18, 2019

What are the Basics of Biochemical Techniques?

Biochemical studies or Biochemical Techniques rely on the availability of appropriate analytical techniques and their applications. This undergraduate course deals with the different biochemical techniques such as spectrophotometry and chromatography to gain knowledge on the biomolecules such as proteins, lipids, carbohydrates, nucleic acids, and their functions. In recent years, huge advances are made in understanding […]

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  • Updated April 12, 2019

Assay of Acid Phosphatase enzyme activity from Potatoes

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using […]

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  • Updated April 12, 2019

Spectrophotometer Instrumentation : Principle and Applications

What are the basic components of Spectrophotometer instrumentation? What is Electromagnetic radiation? Electromagnetic radiation has been put to many uses in our daily routine. The radio and television broadcasting, medical x-ray etc are some common examples. The use of electromagnetic radiation in analytical chemistry gained much importance during the last 50 years for characterization of […]

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  • Updated April 8, 2019