Spectrophotometer Instrumentation : Principle and Applications

What are the basic components of Spectrophotometer instrumentation? What is Electromagnetic radiation? Electromagnetic radiation has been put to many uses in our daily routine. The radio and television broadcasting, medical x-ray etc are some common examples. The use of electromagnetic radiation in analytical chemistry gained much importance during the last 50 years for characterization of […]

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  • Updated April 8, 2019

Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

This is the Biochemistry Protocols section post. Here we are providing the “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know a few points about “Urease Enzyme”. Table of Contents What is Urease?Special characteristic features of Urease Enzyme What is Urease? Urease is a hydrolytic enzyme that catalyzes the […]

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  • Updated April 12, 2019

Effect of Temperature on Amylase activity (Enzymology Protocol)

Table of Contents Aim:Principle:Reagents:Procedure:Report: Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An […]

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  • Updated April 12, 2019

Assay of Salivary Amylase enzyme activity

This article providing the information on “Salivary amylase, functions, and Assay of Salivary amylase enzyme activity”. An enzyme is a protein molecule that is a biological catalyst with three characteristics. The basic function of an enzyme is to increase the rate of a reaction. Most enzymes act specifically with only one reactant, called a substrate, to produce products. Most remarkable characteristic is that enzymes […]

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  • July 28, 2016

What is Zwitter Ion and Isoelectric point

Amino acids have at lease two ionizable groups, i.e., -COOH and -NH2+, of which the former dissociates more easily than the latter. at physiological pH (7.3), the carboxyl group exists in ionized form whereas the amino group remains undissociated and thus retains a positive charge. This doubly charged molecule of amino acid containing a negative […]

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  • Updated April 12, 2019

Heme Synthesis : Synthesis of Porphyrin Molecule

Heme is a Porphyrin molecule. The porphyrins found in nature are compounds in which various side chains are substituted for the 8 hydrogen atoms numbered in Porphin nucleus. These notes explain about Heme synthesis in animals and plants. What is the Composition of Blood? What happens to Blood Sugar after a Meal? Plasma Proteins: Types […]

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  • Updated April 12, 2019