What are the Basics of Biochemical Techniques?

Biochemical studies or Biochemical Techniques rely on the availability of appropriate analytical techniques and their applications. This undergraduate course deals with the different biochemical techniques such as spectrophotometry and chromatography to gain knowledge on the biomolecules such as proteins, lipids, carbohydrates, nucleic acids and their functions. In recent years, huge advances are made in understanding […]

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Assay of Acid Phosphatase enzyme activity from Potatoes

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using […]

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Spectrophotometer Instrumentation : Principle and Applications

What are the basic components of Spectrophotometer instrumentation? What is Electromagnetic radiation? Electromagnetic radiation has been put to many uses in our daily routine. The radio and television broadcasting, medical x-ray etc are some common examples. The use of electromagnetic radiation in analytical chemistry gained much importance during the last 50 years for characterization of […]

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Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

This is the Biochemistry Protocols section post. Here we are providing “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know few points about “Urease Enzyme”. What is Urease ? Urease is a hydrolytic enzyme that catalyzes the Urea into Carbon dioxide and ammonia. The Enzyme Commission Number is 3.5.1.5. […]

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