Effect of Temperature on Amylase activity (Enzymology Protocol)

Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An effect on the stability […]

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Assay of Salivary Amylase enzyme activity

This article providing the information on “Salivary amylase, functions, and Assay of Salivary amylase enzyme activity”. An enzyme is a protein molecule that is a biological catalyst with three characteristics. The basic function of an enzyme is to increase the rate of a reaction. Most enzymes act specifically with only one reactant, called a substrate, to produce products. Most remarkable characteristic is that enzymes […]

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What is Zwitter Ion and Isoelectric point

Amino acids have at lease two ionizable groups, i.e., -COOH and -NH2+, of which the former dissociates more easily than the latter. at physiological pH (7.3), the carboxyl group exists in ionized form whereas the amino group remains undissociated and thus retains a positive charge. This doubly charged molecule of amino acid containing a negative […]

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Heme Synthesis : Synthesis of Porphyrin Molecule

Heme is a Porphyrin molecule. The porphyrins found in nature are compounds in which various side chains are substituted for the 8 hydrogen atoms numbered in Porphin nucleus. This notes explain about Heme synthesis in animals and plants. What is the Composition of Blood ? What happens to Blood Sugar after a Meal? Plasma Proteins: […]

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