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Biochemistry Practical Protocols provided based on UG syllabus.

Assay of Acid Phosphatase enzyme activity from Potatoes

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using […]

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