Archive

Category Archives for "Protocols"

Biochemistry Practical Protocols provided based on UG syllabus.

Estimation of Blood Glucose level by Folin-Wu method

Estimation of Blood Glucose level by Folin-Wu method is one of the clinical biochemistry protocol. You can download this protocols from the given link. Estimation of Blood Glucose level by Folin-Wu method Aim: To estimate Glucose level in Blood by Folin-Wu method Principle: When glucose or other reducing agents are treated with alkaline copper solution […]

Continue reading

Assay of Acid Phosphatase enzyme activity from Potatoes

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using […]

Continue reading

Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

This is the Biochemistry Protocols section post. Here we are providing “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know few points about “Urease Enzyme”. What is Urease ? Urease is a hydrolytic enzyme that catalyzes the Urea into Carbon dioxide and ammonia. The Enzyme Commission Number is 3.5.1.5. […]

Continue reading

Effect of Temperature on Amylase activity (Enzymology Protocol)

Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An effect on the stability […]

Continue reading