Archive

Category Archives for "Quantitative Analysis"

Assay of Acid Phosphatase enzyme activity from Potatoes

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using […]

Continue reading
  • Updated April 12, 2019

Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

This is the Biochemistry Protocols section post. Here we are providing the “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know a few points about “Urease Enzyme”. What is Urease? Urease is a hydrolytic enzyme that catalyzes the Urea into Carbon dioxide and ammonia. The Enzyme Commission Number is […]

Continue reading
  • Updated April 12, 2019

Effect of Temperature on Amylase activity (Enzymology Protocol)

Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An effect on the stability […]

Continue reading
  • Updated April 12, 2019

Assay of Salivary Amylase enzyme activity

This article providing the information on “Salivary amylase, functions, and Assay of Salivary amylase enzyme activity”. An enzyme is a protein molecule that is a biological catalyst with three characteristics. The basic function of an enzyme is to increase the rate of a reaction. Most enzymes act specifically with only one reactant, called a substrate, to produce products. Most remarkable characteristic is that enzymes […]

Continue reading
  • July 28, 2016

Colorimetric Analysis: BRADFORD PROTEIN ASSAY

The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assessing protein concentrations for gel […]

Continue reading
  • Updated July 16, 2017

Determination of Carbohydrate by Anthrone Method

Carbohydrates are a very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides.  The basic units of carbohydrates are Monosaccharides. When hydrolyzing the carbohydrates, gives monosaccharides, but when hydrolyzing monosaccharides it cannot be split into more simpler sugars. The hydrolyzed product of Polysaccharide is estimating […]

Continue reading
  • Updated April 12, 2019