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Category Archives for "Quantitative Analysis"

Assay of Acid Phosphatase enzyme activity from Potatoes

This is the enzymology protocol on “Assay of Acid phosphatase enzyme activity from Potatoes”. Before going to the protocol, we should know few points about phosphatase enzyme. A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using […]

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Assay of Urease Enzyme Activity (Enzymology Practical Protocol)

This is the Biochemistry Protocols section post. Here we are providing “Assay of Urease enzyme activity” protocol. before going to the protocol we need to know few points about “Urease Enzyme”. What is Urease ? Urease is a hydrolytic enzyme that catalyzes the Urea into Carbon dioxide and ammonia. The Enzyme Commission Number is 3.5.1.5. […]

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Effect of Temperature on Amylase activity (Enzymology Protocol)

Aim: To study the effect of temperature on enzyme activity and to determine the optimum temperature. Principle: In any enzyme catalyze reaction the velocity increases with temperature till it reaches the maximum the velocity decreases with further increase of temperature this effect of temperature may be due to several reasons. An effect on the stability […]

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Assay of Salivary Amylase enzyme activity

This article providing the information on “Salivary amylase, functions, and Assay of Salivary amylase enzyme activity”. An enzyme is a protein molecule that is a biological catalyst with three characteristics. The basic function of an enzyme is to increase the rate of a reaction. Most enzymes act specifically with only one reactant, called a substrate, to produce products. Most remarkable characteristic is that enzymes […]

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