Category Archives for "Quantitative Analysis"

Estimation of Proteins by Lowry method

Aim: To estimate the concentration of proteins by Lowry method from the given sample. Principle:  The Blue color developed by the reduction of the Phosphomolybdic-Phosphotungastic components in the Folin-ciocalteau reagent by the amino acids Tyrosine and Tryptophan present in the protein plus the color developed by the biuret reaction of the protein with the color […]

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  • Updated April 4, 2015

Estimation of Tyrosine by Folin-Ciocalteau Method

Estimation of Tyrosine by Folin-Ciocalteau Method: The Folin–Ciocalteu reagent (FCR) or Folin’s phenol reagent or Folin–Denis reagent, also called the Gallic Acid Equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants. It is named after Otto Folin, Vintilă Ciocâlteu, and Willey Glover […]

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  • Updated May 5, 2016