Category Archives for "Quantitative Analysis"

Colorimetric Analysis: BRADFORD PROTEIN ASSAY

The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assessing protein concentrations for gel […]

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Determination of Carbohydrate by Anthrone Method

Carbohydrates are very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides.  The basic units of carbohydrates are Monosaccharides. When hydrolyse the carbohydrates, gives monosaccharides, but when hydrolyse monosaccharides it can not be split into more simpler sugars. The hydrolysed product of Polysaccharide are estimating […]

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Estimation of Proteins by Lowry method

Aim: To estimate the concentration of proteins by Lowry method from the given sample. Principle:  The Blue color developed by the reduction of the Phosphomolybdic-Phosphotungastic components in the Folin-ciocalteau reagent by the amino acids Tyrosine and Tryptophan present in the protein plus the color developed by the biuret reaction of the protein with the color […]

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Estimation of Tyrosine by Folin-Ciocalteau Method

Estimation of Tyrosine by Folin-Ciocalteau Method: The Folin–Ciocalteu reagent (FCR) or Folin’s phenol reagent or Folin–Denis reagent, also called the Gallic Acid Equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants. It is named after Otto Folin, Vintilă Ciocâlteu, and Willey Glover […]

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